MafA and MafB regulate genes critical to beta-cells in a unique temporal manner. Artner I(1), Hang Y, Mazur M, Yamamoto T, Guo M, Lindner J, Magnuson MA, Stein R. Author information: (1)Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA. email@example.com OBJECTIVE: Several transcription factors are essential to. Summaries for MAFA gene (According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL) About This Sectio Transcription factor Maf also known as proto-oncogene c-Maf or V-maf musculoaponeurotic fibrosarcoma oncogene homolog is a transcription factor that in humans is encoded by the MAF gene It has been found that the transcription factors PDX-1, MafA, and BETA2, which bind to the A3, C1, and E1 elements, respectively, synergistically control glucose-regulated transcription of the insulin gene in rat (Zhao et al., 2005), whereas there is no indication of any synergistic effect between PDX-1, MafA, or BETA2 on the human insulin promoter (Docherty et al., 2005)
Useful information about the gene from Entrez. MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-speci fic expression of the insulin gene ( INS; MIM 176730) (Olbrot et al., 2002 ). [supplied by OMIM, Mar 2008] show less MafA was detected in the C1/RIPE3b1 binding complex by using MafA peptide-specific antisera. In addition, MafA was shown to bind within the enhancer region (-340/-91 bp) of the endogenous insulin gene in beta TC-3 cells in the chromatin immunoprecipitation assay. These results strongly suggested that MafA was the beta-cell-enriched component of the RIPE3b1 activator. However, reverse. MAFB (MAF BZIP Transcription Factor B) is a Protein Coding gene. Diseases associated with MAFB include Multicentric Carpotarsal Osteolysis Syndrome and Duane Retraction Syndrome 3 With Or Without Deafness. Among its related pathways are Tacrolimus/Cyclosporine Pathway, Pharmacodynamics and Ectoderm Differentiation Type 1 diabetes is characterised by absolute insulin deficiency caused by autoimmune destruction of the insulin-producing pancreatic beta cells. Patients with this disease require daily injections of insulin or islet transplantation. Because islet
The upregulation of genes that are normally repressed in mature beta cells or transcription factors that are transiently expressed in endocrine progenitors was identified in Mafa KO islets as a hallmark of dedifferentiation. The compromised beta cells in db/db and multiple low-dose streptozotocin mice underwent similar dedifferentiation with expression of Mafb, which is expressed in immature. MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008
Among these genes, we focused our attention on two glucose-responsive transcription factors, MafA and Pdx1, known to regulate genes involved in insulin synthesis and secretion. In particular, studies conducted over the past decade by several groups strongly support that MafA [ 26 ] regulates β cells maturation and the acquisition of glucose-stimulated insulin secretion in vivo [ 33 , 34 , 35. In a large white Caucasian family with insulinomatosis and diabetes mellitus, Iacovazzo et al. (2018) performed exome sequencing and identified a heterozygous missense mutation in the MAFA gene (S64F; 610303.0001) that segregated with disease and was not found in public variant databases.Targeted sequencing of MAFA in a Yugoslavian family with INSDM that was originally reported by Tragl and. MafA, also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog A or RIPE3B1, is a beta-cell-specific and glucose-regulated transcriptional activator for insulin gene expression. MafA may be involved in the function and development of beta-cells as well as in the pathogenesis of diabetes. Suitable sources of nucleic acids encoding. Because MafA is a master regulator of the insulin gene 9, we determined the effect of Kindlin-2 deletion on its expression and found that MafA was strongly detected in the nuclei of control β.
Type 1 (T1D) and type 2 (T2D) diabetes are triggered by a combination of environmental and/or genetic factors. Maf transcription factors regulate pancreatic beta (β)-cell function, and have also been implicated in the regulation of immunomodulatory cytokines like interferon-β (IFNβ1). In this study, we assessed MAFA and MAFB co-expression with pro-inflammatory cytokine signaling genes in. This gene, the ortholog of the human MAFA gene, does not appear to have a genome duplicate. It was assigned the 'a' genome duplicate nomenclature to allow accommodation of zebrafish gene symbols mafa and mafb for the orthologs (and genome duplicates) of human MAF. Expression . All Expression Data 6 figures from 2 publications Cross-Species Comparison. Alliance; Bgee; High Throughput Data.
National Center for Biotechnology Informatio . INS-1 stable cell lines permitting inducible up- or downregulation of this transcription factor were established. MAFA overproduction enhanced and its dominant-negative mutant (DN-MAFA) diminished binding of the. To investigate the role of ATF2 and MafA in the regulation of insulin gene expression in β-cells, MIN6 cells were treated with siRNAs that targeted ATF2 and MafA, respectively. Immunoblotting analysis confirmed that endogenous ATF2 and MafA protein levels were reduced to 50-60% of the level in control siRNA-treated cells by treatment with their respective siRNAs.
Plasmid pAd MafA-I-nGFP from Dr. Douglas Melton's lab contains the insert v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) and is published in Nature. 2008 Aug 27. ():. This plasmid is available through Addgene MafA-Gene: Mafa Location: Chr15:75745490-75748228 bp, - strand Genetic Position: Chr15, 34.82 cM Mutation origin: Germline Transmission: Earliest citation of germline transmission: J:99126: Parent Cell Line: Not Specified (ES Cell) Strain of Origin: Not Specified Mutation. Target genes of both Pdx1  and Mafa  have been reported, with many exocytotic genes regulated by Pdx1, and genes of insulin synthesis and secretion regulated by Mafa. Pdx1 is crucial in pancreas formation [14, 15] and beta cell differentiation [16-18]. A role for Mafa in maturation is supported by: (1) several of its putative target genes (insulin 2, prohormone convertase 1/3 (Pcsk1.
Phenotype data for mouse gene Mafa. Discover Mafa's significant phenotypes, expression, images, histopathology and more. Data for gene Mafa is all freely available for download MAFB (MAF BZIP Transcription Factor B) is a Protein Coding gene. Diseases associated with MAFB include Multicentric Carpotarsal Osteolysis Syndrome and Duane Retraction Syndrome 3 With Or Without Deafness.Among its related pathways are Tacrolimus/Cyclosporine Pathway, Pharmacodynamics and Ectoderm Differentiation
Insulin gene expression is regulated by several islet-enriched transcription factors. However, MafA is the only β cell-specific activator. Here, we show that MafA selectively induces endogenous insulin transcription in non-β cells. MafA was also first detected in the insulin-producing cells formed during the second and predominant phase of β cell differentiation, and absent in the few. MAFA assists in insulin regulation. An in vivo study on mice proved MafA binds to the promoter in an insulin gene to regulate insulin transcription in response to serum glucose levels. MafA is a β cell-specific activator, which differentiates it from other transcription factors involved with insulin gene expression . Uniprot ID Q8NHW3. Protein Name Transcription factor MafA. Ordering Information. A700-067. 100 µl (50+ tests) $389 100 µl (50+ tests) A700-067-T. 10 µl (5+ tests) $103 10 µl (5+ tests) Request Quote for Bulk Quantity. This is a BSA-free Product This product does not contain BSA. Each vial of antibody has. Unlike rat MAFA, expression of the MAFA‐L gene is not limited to mast cells and basophils. In common with other genes in the NK cell gene complex MAFA‐L is also expressed by natural killer cells as well as the monocyte‐like cell‐line U937. Expression in NK cells is restricted to peripheral blood NK cells, decidual NK cells do not express MAFA‐L. While MAFA‐L and rat MAFA might have. MAFA [nucleoplasm] Stable Identifier. R-HSA-21128
MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS).MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM. MafA targets genes essential for metabolism-secretion coupling. Total RNA samples (20 μg) were analysed by hybridising with indicated cDNA probes. The experiments were repeated twice with similar results. a MafA*22 cells were cultured as described in Fig. 3a. Two seperate experiments were blotted side by side to demonstrate the consistency. MafA was induced, respectively, by 3.4-, 8.3-, and. Biological context of MAFA MAFA thus qualifies as a master regulator of beta-cell-specific gene expression and function . There may exist a RIPE3b1 activator binding site in the glucose -responsive element from -1138 to -880 bp . The DNA binding activity of the RIPE3b1 transcription factor of. Mafa - Gen online dizzjunarju fuq Glosbe. Browse 10000 frażijiet u memorji tat-traduzzjoni 10000 . B'xejn MAFA gene product. hMafA, RIPE3b1. MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]). [supplied by OMIM, Mar 2008], , , , More gene data. Featured antibodies. Biorbyt: orb163852: 0 references Polyclonal WB . Antibodies . Compare.
How do you say MAFA (gene)? Listen to the audio pronunciation of MAFA (gene) on pronouncekiwi. Sign in to disable ALL ads. Thank you for helping build the largest language community on the internet. pronouncekiwi - How. Insulin gene expression is regulated by several islet-enriched transcription factors. However, MafA is the only beta cell-specific activator. MafA selectively induces endogenous insulin transcription in non-beta cells. MafA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell. 3.1 MafA is phosphorylated by p38 MAP kinases in vitro and in vivo. Expression vector for HA‐tagged MafA was transiently transfected into 293T cells, together with plasmids encoding p38 α and an activated version of its upstream kinase (MKK6 EE), both tagged with a Flag epitope. Cellular lysates were then analyzed by anti‐MafA immunoblot. recombination of the mafA gene with KOr and PGK-gb2 neo in Escherichia coli; (C) transgene after removal of the PGK-gb2 neo cassette for microinjection; and (D) results of genotyping showing 215 bp products in the transgenic genome encoding Kusabira-Orange. 40 Nishimura et al. lated insulin secretion were not significantly differ- ent between MafA-KOr mice and wild-type mice (Fig. 4E, F). Real.
. 2002; Olbrot et al. 2002; Matsuoka et al. 2003; Artner et al. 2007), and gene ablation of mafA or mafB in mice leads to diabetes (Zhang et al. 2005; Artner et al. 2007; Nishimura et al. 2008). MafA also regulates a set of b-cell-speciﬁc genes, such as glucose transporter 2 (glut2) and granuphilin. Autonomic signaling regulates insulin secretion in response to food intake, a process disturbed in diabetes. Ganic et al. show that the MAFA transcription factor controls autonomic-nervous-system-mediated insulin secretion by regulating nicotinic signaling and identify diabetes risk alleles in human nicotinic receptor genes, suggesting a potential link between smoking and diabetes
Gene name: KLRG1, 2F1, CLEC15A, MAFA, MAFA-2F1, MAFA-L, MAFA-LIKE. Definition (RefSeq) killer cell lectin like receptor G1. KO: K10076 : killer cell lectin-like receptor subfamily G member 1: Organism: hsa Homo sapiens (human) Brite: KEGG Orthology (KO) [BR:hsa00001] 09180 Brite Hierarchies 09183 Protein families: signaling and cellular processes 04091 Lectins [BR:hsa04091] 10219 (KLRG1. HMGA1 Potentiates the Activities of PDX-1 and MafA on INS Gene Transcription. We next determined whether HMGA1, PDX-1, and MafA functionally cooperate to activate INS transcription. HEK-293 cells were cotransfected transiently with Luc reporter plasmids containing human or mouse INS gene promoter and various effector vectors. HEK-293 cells were ideally suited for studying the effects of these. MafA is a basic leucine zipper transcription factor, which controls expression of the insulin gene via a rat insulin promoter element (RIPE) 3b regulatory element . MafA deficient mice exhibit age-dependent glucose intolerance and abnormal islet architecture, suggesting that MafA is primarily involved in β-cell formation and functionality Wada, C., T. Yura 1982. Inhibition of initiation of mini-F plasmid replication in temperature-sensitive mafA mutants of Escherichia coli K-12. Plasmid 8:287-298; Wada, C., T. Yura 1984. Control of F plasmid replication by a host gene: evidence for interaction of the mafA gene product of Escherichia coli with the mini-F incC region. J.Bacteriol.
Mutation details: The loxP flanked segment of Mafa tm1Eyc, extending from 46 bp upstream of the ATG translation initiation site of the endogenous gene to 23 bp downstream of its polyadenylation signal (and encompassing a FRT-flanked PGK-neo cassette), has been deleted by Cre recombinase.This places the coding sequence for nuclear localized E. coli beta-galactosidase (lacZ), which had followed. MafA is a member of the Maf basic leucine-zipper containing transcription factor family. MafA is an islet-enriched protein that plays a role in beta cell-specific and glucose-responsive insulin gene transcription. MafA is expressed exclusively in cell populations that will become islet beta cells. 378435. Mafa . v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian). mafa Gene Set. Dataset: GeneRIF Biological Term Annotations: Category: structural or functional annotations Type: biological term: Similar Terms: Downloads & Tools : Genes. 13 genes co-occuring with the biological term mafa in literature-supported statements describing functions of genes from the GeneRIF Biological Term Annotations dataset. Symbol Name; ATF2 activating transcription factor 2. View all screenings for gene MAFA; Submit new data; MAFA gene homepage. General information; Gene symbol: MAFA: Gene name: v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A: Chromosome: 8: Chromosomal band: q24.3: Imprinted: Unknown: Genomic reference: NC_000008.11: Transcript reference: NM_201589.3: Associated with diseases -Citation reference(s)-Curators (0)-Total number of. MAFA. Sports Club in Mufulira, Zambia. Always Open. SPONSORSHIP OF END SEASON AWARD PRESENTATION. SPONSORSHIP FOR ANNUAL AWARD EVENT - 2019/2020 SEASON - MUFULIRA AMATEUR FOOTBALL ASSOCIATION. I refer... See More. Community See All. 21 people like this. 21 people follow this. About See All. Plot 568 Section 2 Kantanshi (7,609.65 mi) Mufulira, Zambia, +260. Get Directions +260 964037156.
That was the reward Gene Borrello got for helping the feds snag more than 20 Bonanno family cohorts in a series of arsons, murder plots, extortion and home-invasion robberies in his own neighborhood Mafa Tankanhänger Milch isoliert 18 m3 3 Kammern Baujahr 2011 Gesamtgewicht 24000 Leergewicht 5680 Reifen Profil 65 Prozent Mittelachse Liftachse Mercedes Benz Scheibenbremsachsen 1 und 3 Achse 80 Prozent,2 Achse 60 Prozent Luftbälge und Stoßdämpfer neuwertig, EBS Bremssystem Chassi Verzinkt und Lackiert Rücknahme von Altfahrzeugen möglich Europaweite Lieferung möglich Der angezeigte. These results indicate that Pax4 synergistically acts with Pdx1, Ngn3 and MafA to promote the expression of these genes, thereby improving GSID. However, the underlying mechanisms remain to be elucidated. In conclusion, the present study demonstrated that the Pax4 gene was able to synergistically act with the transcription factors Pdx1, Ngn3 and MafA to convert HuMSCs to functional pβCs. We inactivated the mouse c-maf and mafA genes by inserting into these loci marker genes encoding either a normal or nls-tagged b-galactosidase (lacZ), respectively, and we inactivated the mafB gene by inserting into that locus a marker gene encoding gfp. Therefore, the expression of these marker genes could serve as a surrogate for normal expression of the maf genes in the mutant mice. In mafA.
MAFA (C-term) rabbit polyclonal antibody, Aff - Purified Provider product page OriGene - AP52583PU-N Antibody type Polyclonal Description MAFA (C-term) rabbit polyclonal antibody, Aff - Purified Host Rabbit Conjugate Unconjugated Epitope MAFA Antibody clone number NULL Vial size 400 ?l Concentration lot specifi MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMI Human peripheral blood lymphocytes were stained with CD56 PE and anti-human KLRG1 (MAFA) (clone SA231A2) Alexa Fluor® 700 (left) or mouse IgG2a, κ Alexa Fluor® 700 isotype control (right) MafA, Pdx-1, and NeuroD1 also control glucose-regulated transcription of the insulin gene (2-5) (Table 1). MafA was found to interact functionally with Pdx-1 and NeuroD1 to promote synergistic activation of the insulin enhancer-driven reporter cell in non-β-cells ( 84 ) and shown to play a direct and principal role in insulin gene activation in β-cell lines ( 84 ) Generation of Insulin-Producing Cells from the Mouse Liver Using b Cell-Related Gene Transfer Including Mafa and Mafb Haruka Nagasaki1., Tokio Katsumata1., Hisashi Oishi1,2*, Pei-Han Tai1, Yukari.
MafA and MafB are basic leucine zipper transcription factors expressed in mature pancreatic β- and α-cells, respectively. MafA is not only an insulin gene transcription factor but is also. Reaktivität: Human, Maus Wirt: Kaninchen Klon: Polyklonal | MAFA Antikörper (ABIN1911183) Buy mafa antibodies from Santa Cruz Biotechnology, Inc. Monoclonal antibodies are available to most protein immunogens MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene. MAFA cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its ocogenic activity. Serves as an ocogene or tumor supressor depending on the cell context
mafa (ингл. MAF bZIP transcription factor A [d]  ) — кешенең 8-нче хромосомасы [d]  аксымы , шул ук исемдәге ген тарафыннан кодлана торган югары молекуляр органик матдә Mafa- beaux poèmes., Ville De Pétion-Ville, Ouest, Haiti. 8,566 likes · 21 talking about this. *dans l'obscurité, j'avance au clair de ma plume Et si j'écris,c'est pour mettre mes regrets face à mes..